disappeared Any secondary spot in the chromatograrn obtained with solution

minutes and spray with phosphomolybdic acid solution While still damp expose it lo the vapour of strong ammonia solution and examine immediately after the yellow background has disappeared Any secondary spot in the chromatograrn obtained with solution (1) is not more intense than the corresponding spot in the chromatogram obtained with solution (2).Related substances Carry out the method for thin-layer chromatography, Appendix 46. using silica gel G as [he coating substance and dichloromethane as the mobile phase. Apply separately to the plate S ul of each of Two solutions in ether containing (1) 2.5% w/v of the substance being examined and (2) 2.5% w/v of 1H1-dimethylethyl-4-methoxyphenol RS and 0.25% w/v of 2,2-dimelhylethyM-methoxyphenol RS applying each solution in four portions After removal of the plate, allow rt to dry in air and spjay with phosphomolybdic acid solution. While slill damp expose it to the vapour of strong ammonia solution and examine immediately after the yellow background has disappeared Any spots m the chjomatogram obtained with solution (1) other than that corresponding to the 1,1dimethylethyl-4-methoxyphenol RS are not more intense than the spot obtained with 2H2-dimethylethyl-4-methoxyphenol RS in the chromafogram obtained with solution [2).Sufphated ash Not more than 0 05%. Appendix 3.22 Mol Wt 220.35 Butytated Hydroxyloluene is 2,6-bis(1.1 -dimethylelhyl)-4rnethylphenol Category Pharmaceutical aid (anlionidant) Description White lo yellowish while, crystalline powder Solubility Very soluble m acetone, in chloroform and in ether, freely soluble in ethanol (95%). in methane!, in fixed oils and in fats, insoluble in water Storage Store in well-closed containers STANDARDS Identification Test A may be omitted if tests B, C, D and E are earned out Tests 8 and C msy be omitted if tests A, D and E are earned out A The infra-red absorption spectrum, Appends 5.4, is concordant with the reference spectrum of bulylated hydroxytoluene or with the specirum obtained from butylated hydroxytoluene RS.B The tighi absorption in the range 230 to 360 nm of a 0.005% w/v solution in elhanol exhibits a maximum only at aboul 278 nm, absorbante al about 278 nm, between 0.40 and 0.45, Appendix 5.5.C Dissolve about 10 mg in 2 ml of ethanal (96%), add f ml of a 0 1% w/v solution of testosterone propionate in ethanol (95%) and 2 nil of 2M sodium hydroxide