: Mm 5 mg with 60 mg of resorcinol and 2 ml of Sulphuric acid [80%) Heat at 200* for 10 minutes. Cool. pour mlu 200 ml of water

of resorcinol and 2 ml of Sulphuric acid [80%) Heat at 200* for 10 minutes. Cool. pour mlu 200 ml of water and add sufficient 5M Sodium hydroxide to make the mixture just alkaline; an intense green fluorescence is produced D A 5% w/v solution gives the reactions of sodium salts, Appendix 3..1 pH Between 8.0 and 9.2. determined m a 1.0% Wv solution, Appendix 8.11.Specific optical rotation Between +132* To +140°. determined in a 1 % w/v solution in a mixture of equal volumes of methanol and Q 02M sodium carbonate, Appendix B 9 Related substances Carry out The method for thin-layer chromatography, Appendix A B. using silica gel F2S4 as the coating substance (precoated Merck silica gel GO F254 pi ales are suitable) and a mixture of 60 volumes of ethyl acetate, 20 volumes of methanol, 11 volumes of water and 1 volume of strong ammonia solution as the mobile phase Apply separately to the plate 5 ul of each of two solutions of the substance being examined in methanol containing (1) 1 50% w/v and (2) 0 030% w/v After removal of the plate, allow it to dry in air and examine under ultra-violet light (254 nm) Spray with a 1 5% w/v solution of vanillin m sulphunc acid (60%) and heat al 105* for 10 to 15 minutes. By both methods of visualisation, any secondaryspot in the chromalogram obtained with solution (1) is not more intense than the spot m the chromatograrm obtained with solution (2) Water Not more than 40% w/w, determined on 0.6 g. Appendix 3.24. Assay Weigh accurately about 1 g and dissolve in 30 ml of water. Add 30 ml of chloroform and 15 ml of a mixture of 10 volumes of 2M hydrochloric acid and 90 volumes of water, shake and allow to separate Add the chloroform layer to 40 ml of a 20% w/v solution of sodium chloride, shake and allow to separate Repeat the extraction with four quantities, each of 15 ml. of chloroform, combine the chloroform extracts and add sufficient chloroform to produce 100.0 ml Evaporate 25.0 ml, dry the residue at 100° at a pressure of2 kPa, dissolve in 10 ml of dimethylfdrmamide and carry out Method 6 for non-aqueous trtration, Appendix 3.45, usmg 01M letrabutylammomum hydroxide as litrant and thymal blue solution as indicator. Perform a blank determination and make any necessary correction Each mf of 0. IM tetrabutylammonium hydroxide is equivalent lo 0 Q3J73 g of C34H48Na2Q7.CARBENQXQLQNE SODIUM TABLETSCarbenoxolone Tablets Usual strength 50 mg. Storage